NIBSC19/246 is the First WHO International Standard for human VEGF165, manufactured by NIBSC (UK WHO International Laboratory for Biological Standards under MHRA), validated via global multi-laboratory collaborative study published as WHO/BS/2023.2443.
Each lyophilized DIN ampoule net weight 5 mg, contains ~10 μg Sf21-expressed recombinant VEGF165, stabilized with trehalose and human serum albumin in pH 5.2 citrate buffer. Total assigned bioactivity per ampoule is 9,000 IU, traceable to legacy WHO reference reagent 02/286 to guarantee consistent international unitage.
All human-derived raw materials tested negative for HBsAg, anti-HIV and HCV RNA. Lyophilized matrix enables ambient temperature shipment without dry ice cold chain; unopened ampoules store long-term at -20°C, reconstituted solution stable up to 7 days at 4°C, repeated freeze-thaw prohibited. Reconstitution rule: dissolve full contents with 1 mL sterile distilled water; carrier protein buffer is required for serial dilution to minimize wall adsorption loss.
This primary WHO standard has no formal expiry date; accelerated degradation tests confirm long-term bioactivity retention under -20°C storage. Official intended use solely for in-vitro VEGF165 cell-based bioassay calibration; administration to human/animals is strictly forbidden.
All spilled residues, used vials must be autoclaved and disposed as biological waste. No separate COA is provided; full characterization, homogeneity and stability data are fully documented in public WHO collaborative study report. Ideal for anti-VEGF biopharma potency testing, VEGF diagnostic kit validation, GLP lab inter-laboratory comparison and academic angiogenesis research. Authorized global distributors offer import documentation, formal commercial invoices and bulk discounts for biotech, IVD and contract testing labs worldwide.
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