供应美国NIST临床检验材料人肝脏中蛋白质标准物质RM 8461
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Purpose: This Reference Material (RM) is intended to support investigations of peptide and protein identification in complex samples such as those in mass spectrometry-based proteomics. RM 8461 can be used to help assess the confidence of peptide and protein identification within a laboratory or comparability between laboratories or among different measurement approaches. RM 8461 can also be used in the development and validation of new investigative approaches for identifying peptides and proteins.
Description: A unit of RM 8461 consists of a single vial containing approximately 0.5 g of freeze-dried human liver material. 美国NIST临床检验材料
Non-Certified Values: RM 8461 was produced to provide a complex human tissue sample for evaluating the performance of proteomics mass spectrometry instruments coupled to liquid chromatography (LC), software for the identification of proteins and peptides, and development of sample preparation methods [1]. Liver tissue was chosen to provide a “normalized” range of protein abundance and to provide sample complexity to cover the chromatographic “space”. Non-certified values do not provide metrological traceability to the International System of Units (SI) or other higher-order reference system [2]. Non-certified qualitative values of identified proteins present in RM 8461 are listed in Table 1 as UniprotKB [3] accession numbers. 人肝脏中蛋白质标准物质RM 8461
目的：本标准物质（RM）旨在支持复杂样品中肽和蛋白质鉴定的研究，如基于质谱的蛋白质组学中的肽和蛋白质识别。RM 8461可用于帮助评估实验室内肽和蛋白质鉴定的置信度，或实验室之间或不同测量方法之间的可比性。RM 8461也可用于鉴定肽和蛋白质的新研究方法的开发和验证。
描述：一个单位的RM 8461由一个小瓶组成，其中含有大约0.5克冻干的人肝脏材料。
非认证值：生产RM 8461是为了提供一种复杂的人体组织样品，用于评估与液相色谱（LC）耦合的蛋白质组学质谱仪器的性能，用于鉴定蛋白质和肽的软件，以及样品制备方法的开发[1]。选择肝组织是为了提供“标准化”的蛋白质丰度范围，并提供覆盖色谱“空间”的样品复杂性。非认证值不能提供国际单位制（SI）或其他更高阶参考系统的计量可追溯性[2]。RM 8461中存在的已鉴定蛋白质的未经认证的定性值在表1中以UniprotKB[3]登录号列出。