NIST- RM 8323 Yeast Protein Extract

NIST- RM 8323 Yeast Protein Extract


Purpose: Reference Material (RM) 8323 is intended to support measurements used to identify proteins in complex protein mixtures such as those used in proteomics. RM 8323 can be used to help assess measurement repeatability within a laboratory or comparability between laboratories or among different measurement approaches [1]. RM 8323 can also be used in the development and validation of new measurement approaches for identifying proteins in complex protein mixtures. Yeast Protein Extract

Description: A unit of RM 8323 consists of three vials, each containing 200 μL of frozen yeast protein extract solution. The proteins extracted from Saccharomyces cerevisiae have been solubilized in 50 mmol/L ammonium bicarbonate in water.

Non-Certified Values: Non-certified values are suitable for use in method development, method harmonization,and process control but do not provide metrological traceability to the International System of Units (SI) or other higher-order reference system. A spectrophotometric method employing bicinchoninic acid (BCA) was utilized for total protein content measurements [4]. Non-certified mass concentration value for total protein content is provided below. NIST- RM 8323

目的:参考材料(RM)8323旨在支持用于鉴定复杂蛋白质混合物中蛋白质的测量,如蛋白质组学中使用的那些。RM 8323可用于帮助评估实验室内的测量重复性或实验室之间或不同测量方法之间的可比性[1]。RM 8323也可用于开发和验证用于鉴定复杂蛋白质混合物中蛋白质的新测量方法。




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