NIST- SRM 1955a Homocysteine and Folate in Frozen Human Serum
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This Standard Reference Material (SRM) is intended primarily for use in evaluating the accuracy of clinical procedures for the determination of homocysteine and folate (in various forms) in human serum. It is also intended for use in validating working or secondary reference materials. A unit of SRM 1955 consists of three bottles of frozen human serum, each of a different analyte concentration level. Each bottle contains 1 mL of human serum.
The certified concentrations and their expanded uncertainties for total homocysteine (tHCY) and 5-methyltetrahydrofolic acid (5MT) are listed in Table 1. The certified concentrations of tHCY were determined at NIST using a combination of higher-order reference measurement procedures based upon isotope dilution with gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), and liquid chromatography/tandem mass spectrometry (LC/MS/MS) [1–3]. The certified concentrations for 5MT were determined by a combination of reference measurement procedures based upon isotope dilution LC/MS/MS performed at NIST [3,4] and the Centers for Disease Control and Prevention (CDC) [5]. The methods performed at NIST for tHCY and 5MT are recognized as approved higher order reference measurement procedures by the Joint Committee on Traceability in Laboratory Medicine (JCTLM) [6]. The certified concentrations apply only to serum thawed to room temperature, 20 °C to 25 °C (see “Instructions for Storage, Stability, and Use”). NIST- SRM 1955a
NIST通过认证和提供1300多种成分或财产特征良好的标准参考材料®，或两者兼而有之，支持准确和兼容的测量。作为整体质量保证计划的一部分，这些材料用于以单位进行仪器校准，以验证特定测量的准确性，并支持新测量方法的开发。 Homocysteine and Folate in Frozen Human Serum
工业界、学术界和政府使用NIST SRM来促进商业和贸易以及促进研发。目前，NIST SRM可用于工业材料生产和分析、环境分析、健康测量以及科学和计量学的基本测量等领域。NIST SRM也是支持美国测量可追溯性的一种机制。
适用时，每种NIST标准参考材料®均附有分析证书和材料安全数据表。此外，NIST还发表了许多文章和实践指南，描述了SRM的开发、分析和使用。
SRM计划参考了与其SRM和RM的生产、认证和使用有关的许多定义。SRM使用中采用的某些定义来源于参考材料和测量的国际指南和标准，而SRM项目制定的其他定义则描述了NIST运营所特有的活动。
NTRM（NIST可追溯参考材料）是一种商业生产的参考材料，与现有NIST标准具有明确的可追溯性联系。这种可追溯性联系是通过NIST定义的标准和协议建立的。商业参考材料生产商可以在根据这些标准和协议生产的材料上贴上NTRM商标。