NIST- RM 8321 Peptide Mixture for Proteomics
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该参考材料（RM）旨在支持用于鉴定复杂肽混合物中肽的研究，例如基于质谱的蛋白质组学中的肽。RM 8321可用于帮助评估实验室内肽鉴定的置信度或实验室之间或不同测量方法之间的可比性。RM 8321也可用于开发和验证新的研究方法，以鉴定复杂肽混合物中的肽。RM 8321的单位由三个小瓶组成，每个小瓶含有约50µL的冷冻水溶液，其中含有0.1 mL/L甲酸。据估计，该RM中的肽的浓度范围为0.1 pmol/µL至10 pmol/μL。 Peptide Mixture for Proteomics
RM 8321是一种约440种合成肽的水溶液，其浓度范围约为三个数量级。RM 8321旨在提供一种复杂的肽混合物，用于评估与液相色谱（LC）耦合的蛋白质组学质谱仪器的性能[1]。选择肽来覆盖典型的反相梯度洗脱分析的色谱“空间”，提供一系列洗脱图谱。RM 8321中的合成肽与来自50种高丰度人血浆蛋白的胰蛋白酶肽具有相同的氨基酸序列，通过蛋白质组学界发表的多项研究，这些蛋白已被观察为蛋白型肽。蛋白质组肽是由不同的研究人员通过基于质谱的蛋白质组学研究反复观察到的肽。因此，蛋白型肽有望很容易从前体蛋白的酶消化中释放出来，通过电喷雾电离很好地电离，产生高质量的串联质谱（MS/MS）光谱，并且在样品制备和分析过程中是稳定的。 NIST- RM 8321
This Reference Material (RM) is intended to support investigations used to identify peptides in complex peptide mixtures such as those in mass spectrometry-based proteomics. RM 8321 can be used to help assess the confidence of peptide identification within a laboratory or comparability between laboratories or among different measurement approaches. RM 8321 can also be used in the development and validation of new investigative approaches for identifying peptides in complex peptide mixtures. A unit of RM 8321 consists of three vials, each containing approximately 50 µL of frozen aqueous solution containing 0.1 mL/L formic acid. The peptides in this RM are estimated to be in a concentration range of 0.1 pmol/µL to 10 pmol/µL.
RM 8321 is an aqueous solution of approximately 440 synthetic peptides, present at a range of concentrations that span approximately three orders of magnitude. RM 8321 was designed to provide a complex mixture of peptides for evaluating the performance of proteomics mass spectrometry instruments coupled to liquid chromatography (LC) [1].Peptides were chosen to cover the chromatographic “space” of a typical reverse phase gradient elution analysis,offering a range of elution profiles. The synthetic peptides in RM 8321 have the same amino acid sequence as tryptic peptides from 50 high abundance human plasma proteins that have been observed as proteotypic peptides through multiple published investigations by the proteomics community. Proteotypic peptides are those peptides which are observed repeatedly by mass spectrometric-based proteomics investigations by different investigators. Therefore,proteotypic peptides are expected to be readily released from enzymatic digestions of the precursor protein, ionize well by electrospray ionization, generate high quality tandem mass spectrometry (MS/MS) spectra, and are stable during the processes of sample preparation and analysis.