供应美国NIST临床检验材料氨基酸标准物质SRM 2389a
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This Standard Reference Material (SRM) is intended primarily for use in calibration of chromatographic instrumentation for the determination of amino acids. SRM 2389a is a solution of amino acids in a 0.1 mol/L aqueous solution of hydrochloric acid. A unit of SRM 2389a consists of five 2-mL ampoules, each containing approximately 1.2 mL of the solution under argon.
Certified Concentrations of Amino Acids: The certified concentrations and estimated uncertainties for the 16 amino acids provided in Table 1 are based on the results obtained from the gravimetric preparation of the solutions and from the analytical results determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A NIST certified value is a value for which NIST has the highest confidence in its accuracy in that all known or suspected sources of bias have been investigated or taken into account [1]. Values are provided in mass fraction units (milligrams per gram), and, for user convenience, in amount-of-substance concentrations (millimoles per liter) [2].The amount-of-substance concentrations were calculated from the mass fraction values using the density of the solution determined at 20 °C (g/mL) and the relative molecular masses of each amino acid. An allowance for the change in density over the range 18 °C to 23 °C is included in the uncertainty. 美国NIST临床检验材料
本标准参考物质（SRM）主要用于氨基酸测定色谱仪器的校准。SRM 2389a是氨基酸在0.1mol/L盐酸水溶液中的溶液。SRM 2389a的一个单元由五个2毫升的安瓿组成，每个安瓿在氩气下含有大约1.2毫升的溶液。 氨基酸标准物质SRM 2389a
氨基酸认证浓度：表1中提供的16种氨基酸的认证浓度和估计不确定度基于溶液重量制备的结果和使用液相色谱-串联质谱法（LC-MS/MS）测定的分析结果。NIST认证值是指NIST对其准确性具有最高信心的值，因为所有已知或怀疑的偏差源都已被调查或考虑[1]。数值以质量分数单位（毫克/克）提供，为了方便用户，以物质浓度（毫摩尔/升）[2]提供。物质浓度的量是根据质量分数值计算的，使用在20°C（g/mL）下测定的溶液密度和每种氨基酸的相对分子质量。不确定度中包括18°C至23°C范围内密度变化的裕量。