供应美国NIS-SRM 2366a可追溯标准物质巨细胞病毒DNA

供应美国NIS-SRM 2366a可追溯标准物质巨细胞病毒DNA

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This Standard Reference Material (SRM) is intended for use in the value assignment of the number of amplifiable genome copies of cytomegalovirus (CMV) per volume sample. When used to value assign practical CMV calibration materials, SRM 2366a can provide traceability to the International System of Units (SI) for measurement results of CMV viral load in tissues or fluids such as plasma. A unit of SRM 2366a consists of one sterile, 0.5 mL perfluoroalkoxy (PFA) fluoropolymer vial containing approximately 150 μL of extracted DNA solubilized in TE buffer, pH 8.0. TE buffer consists of 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris) and 1 mmol/L ethylenediaminetetraacetic acid (EDTA) tetrasodium salt in deionized water. The CMV DNA is in the form of a bacterial artificial chromosome – known as CMV TowneΔ147 BAC – that contains the genome of the Towne strain of CMV 美国NIS-SRM 2366a

本标准参考物质(SRM)用于分配每个体积样本的巨细胞病毒(CMV)可扩增基因组拷贝数。当用于对实际的CMV校准材料进行赋值时,SRM 2366a可以为组织或液体(如血浆)中CMV病毒载量的测量结果提供国际单位制(SI)的可追溯性。SRM 2366a的一个单元由一个无菌的0.5 mL全氟烷氧基(PFA)含氟聚合物小瓶组成,小瓶中含有约150μL溶解在pH 8.0的TE缓冲液中的提取DNA。TE缓冲液由10 mmol/L 2-氨基-2-(羟甲基)-1,3-丙二醇(Tris)和1 mmol/L组成乙二胺四乙酸(EDTA)四钠盐在去离子水中的溶液。CMV DNA以细菌人工染色体的形式存在,称为CMV TowneΔ147 BAC,其中包含CMV Towne-株的基因组 追溯标准物质

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