NIST- SRM 2365 BK Virus DNA Quantitative Standard

NIST- SRM 2365 BK Virus DNA Quantitative Standard

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This Standard Reference Material (SRM) is intended for use in the value assignment of BK virus deoxyribonucleic acid (DNA) quantitation materials, primarily those used for quantitative polymerase chain reaction (qPCR).SRM 2365 consists of a well-characterized, linearized plasmid, containing BK virus DNA solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3 propanediol hydrochloride (Tris HCl) and 1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) pH 8.0 buffer (TE), with 50 ng/µL yeast tRNA added to ensure stability. A unit of the SRM consists of one 0.5 mL tube containing approximately 110 µL of DNA solution. The tube is labeled and is sealed with a screw cap

本标准参考物质（SRM）用于BK病毒脱氧核糖核酸（DNA）定量材料的赋值，主要用于定量聚合酶链式反应（qPCR）。SRM 2365由一个表征良好的线性化质粒组成，含有溶解在10 mmol/L 2-氨基-2-（羟甲基）-1,3丙二醇盐酸盐（Tris-HCl）和1 mmol/L乙二胺四乙酸二钠盐（EDTA二钠）pH 8.0缓冲液（TE）中的BK病毒DNA，并添加50 ng/µL酵母tRNA以确保稳定性。SRM的一个单元由一个0.5 mL的试管组成，其中含有约110µL的DNA溶液。管道已贴上标签，并用螺帽密封 BK Virus DNA Quantitative Standard

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