NRC CRM-AZA-Mus Lot 200603 is matrix-certified reference material developed by NRC Canada for azaspiracid AZA1, AZA2, AZA monitoring in shellfish, prepared from naturally contaminated Mytilus edulis mussel homogenate. Differing from thermal stabilization, the matrix is sterilized by 17 kGy gamma irradiation to avoid azaspiracid degradation, mixed with multi-antioxidant stabilizers, water content adjusted to 85 %. Each 8 g nitrogen-flushed polypropylene vial is packed in trilaminate light-proof pouch for long-term preservation.
Certified Mussel Matrix CRM for Azaspiracid Toxins Certified values: AZA1 1.16 ± 0.10 mg/kg, AZA2 0.273 ± 0.024 mg/kg, AZA3 0.211 ± 0.023 mg/kg, matching EU regulatory limit of 0.16 mg/kg total AZAs. Certification relies on two independent workflows: exhaustive solid-liquid extraction & MSPD matrix solid phase dispersion, both coupled with LC-MS quantification, with isomer peak area correction integrated into certified results. Full homogeneity test covers between-batch & within-bottle samples, all uncertainty components (characterization, homogeneity, stability) fully calculated with coverage factor k=2 (95% confidence).
Certified Mussel Matrix CRM for Azaspiracid Toxins Isochronous stability study confirms no significant toxin loss at 18 °C for two weeks during shipment; 12-month stable storage at ≤-12 °C. The matrix naturally contains AZA4–12, okadaic acid, dinophysistoxins, yessotoxins and other lipophilic marine biotoxins, with standard LC-MS chromatograms under multiple mobile phases supplied for isomer separation and matrix interference evaluation.
NRC CRM-AZA-Mus Valid for 1 year unopened under -12 °C storage. Equilibrate to room temperature and vortex ≥2 min before use; minimum subsample weight 2 g, avoid repeated freeze-thaw cycles. Azaspiracids are potent gastrointestinal toxins; full PPE and autoclave waste treatment required. Full original COA with irradiation, homogeneity & stability data accepted by global food accreditation bodies. Authorized distributors provide cold-chain customs clearance and formal commercial invoices for bulk lab orders.
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