NIST- SRM998 Angiotensin I (Human)
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This Standard Reference Material (SRM) is intended primarily for use in the calibration and standardization of the renin assay and as a reference peptide for amino acid analysis and high performance liquid chromatography (HPLC).A unit of SRM 998 consists of 0.5 mg of chemically synthesized Angiotensin I.The certified angiotensin I purity value was assessed by HPLC and confirmed indirectly within the reported uncertainty by nuclear magnetic resonance (NMR). In the HPLC procedure, the peptide content was measured by the method of standard additions using phenylalanine dissolved in 1 mol/L KOH as the added standard. The solution of the SRM and added standard was hydrolyzed in 6 mol/L HCl and the amino acid composition was determined. The phenylalanine concentration was determined by absolute weight and by measuring the absorbance of the standard solution and calculating the concentration using an apparent specific molar absorbance of 209 ± 0.2 L/g•cm. The ratio of the concentration of phenylalanine to each of four amino acids (valine, leucine, histidine, and arginine) was used to calculate the amount of total peptide in each sample. All reported uncertainties are stated as plus or minus one standard error of the listed value. The reference acetate content value was determined by a single method. NIST- SRM998
本标准参考物质（SRM）主要用于肾素测定的校准和标准化，并作为氨基酸分析和高效液相色谱（HPLC）的参考肽。SRM 998的一个单位由0.5 mg化学合成的血管紧张素I组成。通过HPLC评估经认证的血管紧张肽I纯度值，并在通过核磁共振（NMR）报告的不确定度。在HPLC程序中，使用溶解在1mol/L KOH中的苯丙氨酸作为添加的标准品，通过标准添加方法测量肽含量。将SRM和添加的标准品的溶液在6mol/L HCl中水解，并测定氨基酸组成。苯丙氨酸浓度通过绝对重量和测量标准溶液的吸光度来确定，并使用209±0.2 L/g•cm的表观比摩尔吸光度计算浓度。苯丙氨酸与四种氨基酸（缬氨酸、亮氨酸、组氨酸和精氨酸）的浓度之比用于计算每个样品中总肽的量。所有报告的不确定性均表示为所列值的正负一个标准误差。参考乙酸盐含量值通过单一方法测定。 Angiotensin I (Human)
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