| Product description | A/Singapore/1/57 (H2N2) Influenza Virus Antigen/A/新加坡/1/57（H2N2）流感病毒抗原/ A/新加坡/1/57（H2N2）流感病毒抗原标准品/单向扩散测定用流感病毒抗原标准品A/新加坡/1/57（H2N2） |
| Product number | 00/548 |
| Category | Vaccines Influenza |
| Keywords | Influenza antigen potency reagent HA |
| Type of standard | Influenza |
| Instructions for Use | 00-548.pdf Instructions for Use |
| Minimum quantity | 1 |
A/Singapore/1/57 (H2N2) Influenza Virus Antigen UNITAGE The potency of 00/548 is 50 µg/ml.
00/548 CONTENTS Country of origin of biological material: United Kingdom. Antigen reagent 00/548 is prepared from formalin-inactivated, partially purified A/Singapore/1/57 (H2N2) virus which was suspended in PBSA buffer containing 1% (w/v) sucrose and processed for freeze-drying in 1ml volumes as described by Campbell, P.J., Journal of Biological Standardisation,1974, 2, 249- 267.
STORAGE Unopened ampoules should be store at -20°C but storage of reconstituted reagent is not recommended. Please note because of the inherent stability of lyophilized material, NIBSC may ship these materials at ambient temperature
USE OF MATERIAL No attempt should be made to weigh out any portion of the freezedried material prior to reconstitution For all practical purposes each ampoule contains the same quantity of the substances listed above. Reconstitute the total contents of one ampoule of Reagent in 1ml of distilled water. Allow to stand for a minimum of 5 minutes before use to allow for complete solution of freeze-dried material. Antigen reagent 00/548 should be used according to the method described by Wood JM, Schild GC, Newman RW and Seagroatt VA. Journal of Biological Standardisation, 1977, 5, 237-247, with the following modification: it is recommended that Antigen Reagent 00/548 and test A/Singapore/1/57 test antigens should be treated with Zwittergent 3-14 detergent (Calbiochem-Behring, La Jolla, CA, USA) before single-radial-diffusion assay. Suitable incubation conditions are as follows: 450µl of antigen are added to 50µl of 10% (w/v) Zwittergent detergent and incubated in covered containers for 30 minutes at room temperature (20-25°C). Dilutions of detergent treated antigens are then added to wells in single radial diffusion immunoplates and incubated at 20-25°C. Antigen Reagent 00/548 should be used to assay A/Singapore/1/57 antigens using antiserum Reagent 00/440.
